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Ureido-2,2´-diacetic acid bis(hydroxysuccinimide) ester is the shortest version of MS-cleavable urea-based lysine-lysine reactive homobifunctional cross-linkers. At neutral or slightly basic pH, it irreversibly crosslinks the neighbouring lysine groups. The presence of the symmetrical urea moiety which is prone to collision-induced dissociation allows to perform unambiguous distinguishing of crosslinks in tandem CID-MS experiments.
The urea-based MS-cleavable crosslinkers are based on the concept pioneered by Prof. Dr. Andrea Sinz of the Halle University.